Characterization of the distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds by SR-FTIR

Abstract Background Bone-tendon interface (enthesis) plays a pivotal role in relaxing load transfer between otherwise structurally and functionally distinct tissue types. Currently, decellularized extracellular matrix (DEM) from enthesis provide natural three-dimensional scaffold with tissue-specific orientations of molecules for regeneration, however, the distributions collagen PGs content book-shaped scaffolds rabbit rotator cuff by SR-FTIR have not been reported. Methods Native tissues (NET) harvested were sectioned into cuboid (about 30 mm × 1.2 10 mm) decalcification. The intrinsic ultrastructure evaluated histological staining scanning electron microscopy (SEM), respectively. also measured innovatively SR-FTIR. Results successfully obtained. Histomorphology SEM effect decellularization structure during decellularization. After mechanical testing, failure NET group showed significantly higher than that DEM ( P < 0.05). Meanwhile, stiffness was lower group. Furthermore, decreased obviously after quantitative analysis. Conclusion applied to characterize morphology native cuff. Moreover, this technology can be mapping distribution scaffolds.